Protein Interaction and Transport Maps of Live Cell Nuclei Using Fluorescence Correlation Spectroscopy in a Single Plane Illumination Microscope
نویسندگان
چکیده
Typical microscopic methods used for characterizing intracellular protein mobility are, e.g., fluorescence photobleaching recovery (FRAP) and fluorescence correlation spectroscopy (FCS). Of these, FRAP can image protein mobility in entire two-dimensional sections of live cells, but is typically limited to the time resolution of confocal image series, some frames per second. FCS, on the other hand, has fast time resolution but so far has been limited to single-point measurements in the focus of a laser beam. Although we have demonstrated first protein mobility maps by point-to-point FCS (1), this method is extremely time-consuming and not very feasible for live cell measurements.
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